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KMID : 0616620000060020327
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Journal of Soonchunhyang Medical College
2000 Volume.6 No. 2 p.327 ~ p.342
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The Protective Effects of Calcium Channel Blocker(Verapamil) on Ischemic and Reperfused Injury in a Rabbit Lung Transplantation Model -An experimental study in isolated rabbit lung model-
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Abstract
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The optimum method for lung preservation in preparation for transplantation remains unresolved. In this study, we evaluated the effectiveness of one pharmacologic agent for prolonging the preservation time of the ischemic lung. We investigated a verapamil which is both a calcium channel blocker and a vasodilator, in regard to its effectiveness in promoting functional recovery of the ischemic rabbit lung in isolated rabbit lung model.
This study was undertaken to investigate whether verapamil administered during pre-ischemic and post-ischemic periods would ameliorate ischemia- reperfusion injury in preserved isolated rabbit lung.
Two groups were evaluated with twelve rabbits in each group.
In the control group, verapamil was not used. In the experimental group, verapamil (0.1mg/kg) was injected intravenously, and then Euro-collins solution 150㎖ (E-C sol. 500㎖ added to verapamil 2cc) was perfused through main pulmonary artery. The left lung was harvested and simply immersed in storage solution (Modified Euro-collins´ solution) for six hours. Before reperfusion, verapamil (0.1mg/kg) was injected through main pulmonary artery.
For assessment, the stored lung was ventilated mechanically and perfused with fresh venous blood at a rate of 40㎖/min for 60 minutes.
① Systemic arterial oxygen tension was decreased in both group during reperfusion, but was significantly higher in the verapamil-treated group compared to control group. (p = 0.0001 - 0.0004)
② Pulmonary arterial pressure was increased in both group during reperfusion, but significantly lower in verapamil-treated group. (p = 0.0001)
③ Airway pressure was increased in both group during reperfusion, but significantly lower in verapamil-treated group from reperfusion 30 minutes to repefusion 60 minutes.(p < 0.0309)
④ Wet/dry weight ratio and water content of the preserved lung were significantly lower in verapamil-administered group than the control group.
⑤ Microscopically, the intra-alveolar hemorrhage and pulmonary congestion was significantly less in the verapamil group. In conclusion, through the use of isolated rabbit lung model, Ca++-channel blocker verapamil prevented injury caused by lung ischemia and reperfusion
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KEYWORD
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